Metal-organic framework-based nanomaterials as opto-electrochemical sensors for the detection of antibiotics and hormones: A review

• Akeem Adeyemi Oladipo,
• Saba Derakhshan Oskouei and
• Mustafa Gazi

Beilstein J. Nanotechnol. 2023, 14, 631–673, doi:10.3762/bjnano.14.52

• (ELISA), radioimmunoassay (RIA), surface-enhanced Raman spectroscopy (SERS), and capillary electrophoresis are common analytical techniques used to qualitatively or quantitatively determine pharmaceuticals in various matrices because they are sensitive (Figure 2), have a significant tolerable limit of

Design of surface nanostructures for chirality sensing based on quartz crystal microbalance

• Yinglin Ma,
• Xiangyun Xiao and
• Qingmin Ji

Beilstein J. Nanotechnol. 2022, 13, 1201–1219, doi:10.3762/bjnano.13.100

• resonance [10], Fourier transform infrared spectrometry (FTIR) [11], UV–vis absorption spectrometry [12], mass spectrometry (MS) [13], titration microcalorimetry [14], high-performance liquid chromatography (HPLC) [15], gas chromatography (GC), capillary electrophoresis (CE) [16], and electrochemical chiral

DNA aptamer selection and construction of an aptasensor based on graphene FETs for Zika virus NS1 protein detection

• Nathalie B. F. Almeida,
• Thiago A. S. L. Sousa,
• Viviane C. F. Santos,
• Camila M. S. Lacerda,
• Thais G. Silva,
• Rafaella F. Q. Grenfell,
• Flavio Plentz and
• Antero S. R. Andrade

Beilstein J. Nanotechnol. 2022, 13, 873–881, doi:10.3762/bjnano.13.78

• ligands by exponential enrichment” (SELEX). In this study, we select an aptamer (termed ZIKV60) by capillary electrophoresis SELEX (CE-SELEX) to the Zika virus non-structural protein 1 (NS1) and counterselection against the NS1 proteins of DENV (serotypes 1, 2, 3, and 4) and YFV. The ZIKV60 dissociation

• reported in an attempt to reduce processing time, generate aptamers with novel designs and functions, or increase the process throughput [14][15][16]. In particular, the CE-SELEX technique is a SELEX variation in which capillary electrophoresis (CE) is used to separate oligonucleotides that have bound to

• incubated with 80 pmol of the ZIKV NS1 protein in borate buffer (60 mM) for 30 min, 200 rpm, at 37 °C. An incubation solution sample was submitted to separation by CE using a neutral capillary on a P/ACE MDQ capillary electrophoresis system (Beckman Coulter Inc., Fullerton, CA). The capillary was first

The different ways to chitosan/hyaluronic acid nanoparticles: templated vs direct complexation. Influence of particle preparation on morphology, cell uptake and silencing efficiency

• Arianna Gennari,
• Julio M. Rios de la Rosa,
• Erwin Hohn,
• Maria Pelliccia,
• Enrique Lallana,
• Roberto Donno,
• Annalisa Tirella and
• Nicola Tirelli

Beilstein J. Nanotechnol. 2019, 10, 2594–2608, doi:10.3762/bjnano.10.250

• was converted into ζ-potential values by means of the Smoluchowski equation using Malvern Zetasizer software (v7.11). Capillary electrophoresis. Electrophoresis measurements were performed at 25 °C on a P/ACE MDQ Plus (SCIEX, Warrington, UK) equipped with a 50 cm effective length (70 cm total length

• free HA after nanoparticle preparation. Left: The HA content of the different nanoparticle formulations (1 mg/mL, deionized water) was measured by capillary electrophoresis before and after dialysis (MWCO = 1,000 kDa). Results are expressed as the relative amount of complexed HA (incorporated in the

Structural and optical properties of penicillamine-protected gold nanocluster fractions separated by sequential size-selective fractionation

• Xiupei Yang,
• Zhengli Yang,
• Fenglin Tang,
• Jing Xu,
• Maoxue Zhang and
• Martin M. F. Choi

Beilstein J. Nanotechnol. 2019, 10, 955–966, doi:10.3762/bjnano.10.96

• lowest separation resolution. Since the stationary phase used in size exclusion chromatography (SEC) has a high surface area, NCs with high surface activity can be irreversibly adsorbed by the column packing material, which limits its application to the separation of NCs. For capillary electrophoresis

• nanoparticles (NPs). Although dialysis can be a useful means of removing impurities from NPs, it is a very time-consuming technique. Capillary electrophoresis provides an efficient and simple technique to fractionate water-soluble NCs [19], however it often depends on the specific core or ligand shell

False positives and false negatives measure less than 0.001% in labeling ssDNA with osmium tetroxide 2,2’-bipyridine

• Anastassia Kanavarioti

Beilstein J. Nanotechnol. 2016, 7, 1434–1446, doi:10.3762/bjnano.7.135

• properties of the degradation products via nanopore translocation can be evaluated to assure base calling quality in a DNA sequencing effort. Keywords: α-hemolysin; capillary electrophoresis; DNA sequencing; high-performance liquid chromatography; nanopores; osmium tetroxide bipyridine; osmylated oligos

• (HPLC) or capillary electrophoresis (CE). It turns out that both analytical tools clearly resolve DNA from the OsBp label, which appears as a single peak with the methods used in this report. For the more typical cases where the oligo/DNA has 5 or more pyrimidines, the resolution between intact and

• unnecessarily long. Protocol A results in over 90% T osmylation and less than 8% C-osmylation and protocol B results in 100% T + C osmylation. Capillary electrophoresis methods and analyses Analyses of the reaction mixtures were carried out using an Agilent 1600 CE instrument equipped with a diode array

Single pyrimidine discrimination during voltage-driven translocation of osmylated oligodeoxynucleotides via the α-hemolysin nanopore

• Yun Ding and
• Anastassia Kanavarioti

Beilstein J. Nanotechnol. 2016, 7, 91–101, doi:10.3762/bjnano.7.11

• dA10dCdA9 is monitored by capillary electrophoresis (CE) analysis. Any side-reaction, or any backbone degradation, or oxidative damage would have been detectable as additional peaks with typical detectability of 0.1%. Any product formed by these processes is expected to retain some nucleotides, and

• pore we also tested the later with a dA25 tail added either to the 3’- or the 5’-end (Table 1). The oligos used in this study were purchased from Integrated DNA Technologies, as PAGE-purified and desalted materials (Table 1). They were analyzed by capillary electrophoresis (CE) to assess purity, and

• ; the peak height of both oligo and osmylated oligo reach 120 mAU. Capillary electrophoresis (CE) overlapping traces of oligodeoxynucleotides pGEX3’-dA25 intact and pGEX3’-dA25 at R1 and R2 levels of osmylation per protocols A and B, respectively (Experimental section, Part A). Materials are at